Certain frog species lay their eggs in stable foams, known as foam nests, which enable the tadpoles to develop outside a body of water. These biofoams can remain stable for weeks, providing all the necessary conditions for tadpole development. Research into their composition have revealed a variety of proteins, including surfactant proteins that reduce water’s surface tension, a characteristic that is rare in nature. Our findings have uncovered a “black box” of proteins that are currently unidentifiable due to the lack of databases related to the genes responsible for these nests. In this project, we plan to employ a proteogenomic approach, which merges proteomic and genomic data to annotate these proteins and understand their influence on the shape and architecture of these biostructures. We aim to uncover insights about the evolutionary history of these species, with potential applications in biotechnology.