Cell membranes are essential barriers that protect and define cells. These membranes comprise a complex lipid matrix; most exhibit a fascinating property called asymmetry. This means that the lipids in the outer layer of the membrane are very different from those in the inner layer. It’s as if nature has carefully selected specific lipids with different properties to meet the challenges of the external environment while using different lipids to meet the needs of the cell’s interior. These different layers are coupled together to form the lipid bilayer. Oxidative processes can damage various cellular components like DNA, proteins, and lipids. Interestingly, polyunsaturated lipids, mainly found in the inner layer of cell membranes, are particularly susceptible to oxidation. This research aims to investigate how lipid oxidation affects the mechanical properties of cell membrane models, considering the complexity of their asymmetric nature.